Experimental Evidences
| Abbreviation | |
| BPP | Binding of Purified Proteins |
| BCE | Binding of Cellular Extracts |
| SM | Site Mutation |
| GEA | Gene expression analysis |
| AS | Author Statement |
| IEP | Inferred from Expression Pattern |
| GEA | Gene expression analysis |
| IMP | Inferred from mutant phenotype |
| IDA | Inferred from Direct Assay |
| IGI | Inferred from Genetic interaction |
| RBM | Reaction Blocked in Mutant |
| APPH | Assay of Protein Purified to Homogeneity |
| MIC | Microarrays |
| qRT-PCR | mRNA expression levels measured by qRT-PCR technique |
| OHRs | One Hybrid Reporter system |
| CHS | ChIP-seq |
| PRM | Primer Extension Analysis |
About EnTRaF
The DNA-binding transcription factors (TFs) have a fundamental role to contend with environmental changes in all the organisms. These proteins affect gene expression positively and/or negatively depending on operator context and ligand-binding status. Prediction and characterization of novel TFs generally involve finding a function for a protein considering a reference dataset (golden standard), which include the information of well-annotated proteins with experimental evidence.
ENTRAF provides a centralized, web-based location to organize information about 1784 TFs with experimental evidence, from bacteria and archaea organisms, collected from Swissprot, PDB, RegulonDB, RegulomeDB, CollecTF, and DBTBS.
ENTRAF version 2.0 contains 2787 references associated with 1784 Transcription factors (TFs). From these, 1712 TFs have been characterized in 29 bacterial orders, such as Gammaproteobacteria, Bacilli, and Actinomycetota; and 72 TFs in ten archaeal orders, as Thermoprotei and Thermococci.